Isothermal DNA amplification with gold nanosphere-based visual colorimetric readout for herpes simplex virus detection.

نویسندگان

  • Eric Tan
  • Barbara Erwin
  • Shale Dames
  • Karl Voelkerding
  • Angelika Niemz
چکیده

References 1. Tenover FC. Rapid detection and identification of bacterial pathogens using novel molecular technologies: infection control and beyond [Review]. Clin Infect Dis 2007;44:418–23. 2. Higuchi R, Dollinger G, Walsh PS, Griffith R. Simultaneous amplification and detection of specific DNA sequences. Biotechnology 1992;10:413–7. 3. Kost CB, Rogers B, Oberste MS, Robinson C, Eaves BL, Leos K, et al. Multicenter beta trial of the GeneXpert enterovirus assay. J Clin Microbiol 2007;45:1081–6. 4. Nurmi J, Korpimäki T, Lövgren T, Kojola H, Iitiä A. Temperature control of reaction vessel, system with reaction vessel, software product for system and use of system. European patent application EP1771250, 2004. 5. Korpimäki T, Lövgren T, Nurmi J. Method for stabilizing assay reagents, reagent container with stabilized assay reagents and use thereof. European patent application EP1766055, 2004. 6. Steere AC. Lyme disease [Review]. N Engl J Med 1989;321:586–96. 7. Kiviniemi M, Nurmi J, Turpeinen H, Lövgren T, Ilonen J. A homogeneous high-throughput genotyping method based on competitive hybridization. Clin Biochem 2003;36:633–40. 8. Ylikoski A, Elomaa A, Ollikka P, Hakala H, Mukkala VM, Hovinen J, et al. Homogeneous time-resolved fluorescence quenching assay (TruPoint) for nucleic acid detection. Clin Chem 2004;50:1943–7. 9. Schwaiger M, Peter O, Cassinotti P. Routine diagnosis of Borrelia burgdorferi (sensu lato) infections using a real-time PCR assay. Clin Microbiol Infect 2001;7:461–9. 10. Nurmi J, Wikman T, Karp M, Lövgren T. High-performance real-time quantitative RT-PCR using lanthanide probes and a dual-temperature hybridization assay. Anal Chem 2002;74:3525–32. 11. von Lode P, Rosenberg J, Pettersson K, Takalo H. A europium chelate for quantitative point-of-care immunoassays using direct surface measurement. Anal Chem 2003;75:3193–201. 12. Nurmi J, Syrjälä A, von Lode P, Hagren V. Method for the detection of the presence of a target polynucleotide in a sample. Patent application 20070203, 2007.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Loop-Mediated Isothermal Amplification (LAMP) for the Rapid Diagnosis of Herpes Simplex Virus Type 1 (HSV-1)

Background and Aims: considering difficulties in usual laboratory methods in detection of viral infections, improved DNA-based diagnostic techniques are more reliable. Loop mediated isothermal amplification method (LAMP) is a nucleic acid amplification method that amplifies DNA using six primers which has been developed to diagnose viruses as a rapid and high efficiency test. In this study, the...

متن کامل

Localization of Herpes Simplex Virus Type 1 DNA in Latently Infected BALB/c Mice Neurons Using in situ Polymerase Chain Reaction

Background: Herpes simplex virus type-1 (HSV-1) establishes a lifelong latent infection in neurons following primary infection. The existence of latent HSV-1 DNA in the trigeminal ganglia of infected BALB/c mice was examined using a direct in situ PCR technique, based on Digoxigenin-11-dUTP detection system with anti-digoxigenin-peroxidase and 3,3'-diaminobenzidine (DAB) substrate. Methods: Eig...

متن کامل

Determination of Herpetic Keratitis Frequency Using Loop Mediated Isothermal Amplification (LAMP) Method in Isfahan, Iran

Herpetic keratitis which is resulted from herpes simplex virus type I, is considered the most common cause of corneal blindness that needs being diagnosed and treated in order to prevent irreversible eye effects. The best method to diagnose it is molecular method which has high sensitivity and exclusivity. The current study was done on 25 eye samples collected from patients referred to the Feyz...

متن کامل

Determination of Herpetic Keratitis Frequency Using Loop Mediated Isothermal Amplification (LAMP) Method in Isfahan, Iran

Herpetic keratitis which is resulted from herpes simplex virus type I, is considered the most common cause of corneal blindness that needs being diagnosed and treated in order to prevent irreversible eye effects. The best method to diagnose it is molecular method which has high sensitivity and exclusivity. The current study was done on 25 eye samples collected from patients referred to the Feyz...

متن کامل

PCR detection of thymidine kinase gen of latent herpes simplex Virus type 1 in mice trigeminal ganglia

  Herpes simplex virus type 1 establishes a latent infection in the peripheral nervous system following primary infection. During latent infection, virus genome exhibit limited transcription, with the HSV LATs consistently detected in latency infected ganaglia. Following ocular infection viral latency develops in the trigeminal ganglia. In this study PCR has been used for detection of HSV-1 nuc...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
  • Clinical chemistry

دوره 53 11  شماره 

صفحات  -

تاریخ انتشار 2007